Abstract: Any cell behave abnormal, like in a disease condition there is a change in it’s metabolism. The Extra cellular Flux analyzer measures these changes by analyzing both the pathways oxidative phosphorylation and glycolytic pathway. The technology looks into the a new dimension “Spare respiratory capacity” to reveal the cell metabolism. With close to 2500 installations and about 20,000 users across the globe, today XF analyzers are used in most of the mammalian biologies with high focus to Immunology, Neuroscience, Cancer, Stem Cell Biology, Metabolic disorders and infectious Biology. Even the systems are used for model Organisms like C-elegans, Drosphila, Zebra fish and very few experimented in plant biology.