Sunil Tripathi (2nd year PhD scholar) presented his seminar on the topic "Neuronal Plasticity and Brain repair mechanism".
R Sumitha (2nd year PhD Scholar) presented her seminar on "Signaling Pathways in Stem Cells and Cancer Cells"
Rukmani (1st year MPhil Scholar) presented the paper by Kim JY etal 2012 from World Journal of Stem Cells entitled: "Umbilical cord blood mesenchymal stem cells protect amyloid-β42 neurotoxicity via paracrine".
AIM: To understand the neuroprotective mechanism of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) against amyloid-β42 (Aβ42) exposed rat primary neurons.
METHODS: To evaluate the neuroprotective effffect of hUCB-MSCs, the cells were co-cultured with Aβ42-exposed rat primary neuronal cells in a Transwell apparatus. To assess the involvement of soluble factors released from hUCB-MSCs in neuroprotection, an antibody-based array using co-cultured media was conducted. The neuroprotective roles of the identified hUCB-MSC proteins was assessed by treating recombinant proteins or specific small interfering RNAs (siRNAs) for each candidate protein in a co-culture system.
RESULTS: The hUCB-MSCs secreted elevated levels of decorin and progranulin when co-cultured with rat primary neuronal cells exposed to Aβ42. Treatment with recombinant decorin and progranulin protected from Aβ42-neurotoxicity in vitro. In addition, siRNA-mediated knock-down of decorin and progranulin production in hUCB-MSCs reduced the anti-apoptotic effffects of hUCB-MSC in the co-culture system.
CONCLUSION: Decorin and progranulin may be involved in anti-apoptotic activity of hUCB-MSCs exposed to Aβ42.
Vrinda M (3rd year PhD Scholar) presented the paper by Hung etal 2011 from Epilepsy Research entitled: "The duration of sustained convulsive seizures determines the pattern of hippocampal neurogenesis and the development of spontaneous epilepsy in rats"
The duration of sustained seizures (SS) plays a crucial role in the occurrence of spontaneous recurrent seizures (SRS) in experimental animals. We tested whether rats with varying durations of initial convulsive SS exhibited differential neurogenesis patterns in the hippocampal dentate gyrus that may be related to subsequent epileptogenesis. Sprague-Dawley rats with pilocarpine-induced convulsive SS were divided into short SS (30 min) and long SS (2 h) groups. Their behavior was monitored to identify convulsive SRS. From 1 to 28 days post-SS, cell proliferation was evaluated by 5'-bromo-2'-deoxyuridine (BrdU) labeling and immature neuroblasts in the dentate gyrus were identified by doublecortin immunohistochemistry.Convulsive SRS was detected in 8 out of the 9 long SS rats, but not in the 9 short SS rats. During day 1-3, proliferative cells were diffusely localized throughout the hippocampus in the long SS rats but were primarily confined within the subgranular zone in the short SS rats. Within the subgranular zone, a significant increase in the number of BrdU-positive cells was found at days 3 and 7 after the long SS and on day 1 after the short SS. Notably, abnormal dendritic outgrowth and hilar-ectopic localization of doublecortin-positive cells were present in the long SS rats. In conclusion, aberrant hippocampal neurogenesis following long SS may contribute to the development of SRS.
Poojashri Mishra (3rd year PhD scholar) presented a seminar on Neurodegeneration and carbohydrates. She explored the Hexose biosynthesis pathways and the role of nutrients in pro-survival and pro-growth activity.
Glycosylation (and O-GlcNac) is involved in formation of vesicular proteins, in the nucleolus (both transcriptional and translational activity), and with tau proteins, neurofilaments and tubulins. GlcNac is implicated in Type II diabetes and seems to be reduced in Cancer and Neurodegenerative diseases such as MS, ALS etc. Glucosamine seems to inhibit Lipopolysaccharides (that activate microglia).
Glucosamine injection given in the peri-infarct period (1 hr pre to 3 hr post) seems to be neuroprotective.